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- 详细信息
- 文献和实验
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- 供应商:
Neobioscience
- 保存条件:
-20
- 规格:
100 µL
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文献和实验When trying to identify a clone within a cDNA library, which may contain a coat protein (CP) gene, one useful technique may by immunological screening, using antibodies raised against either purified virus or isolated CP. Antibody screening
: aliquot a portion of the stock to a separate tube and dilute to OD600 = 0.5-0.7 using 10mM MgSO4 PROCEDURE - Step 2 - Determine Library titer and Library plating 1. Grow ON culture of Y
The Design, Annotation, and Application of a Kinase-Targeted Library
We present here a workflow for designing a kinase-targeted library (KTL) with the goal of capturing known kinase inhibitor chemical space. We validated our design retrospectively using recent, high-throughput screening data
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