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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Taq DNA Polymerase, GMP Grade
- 供应商:
嵘崴达
- 保质期:
见产品外包装
- 保存条件:
−20°C
- 库存:
需确认
- 规格:
1000 units
Taq DNA Polymerase, GMP Grade
5 U/βl
别名: dna amplification, pcr, polymerase, primer extension
General description
Taq DNA Polymerase, GMP Grade, is manufactured under GMP conditions. It also fulfills the high quality and documentation requirements of research and development laboratories in the pharmaceutical and biotechnology industries. Taq DNA Polymerase, GMP Grade, gives high lot-to-lot consistency. Recombinant Taq DNA Polymerase (from Thermus aquaticus, recombinant E. coli), GMP Grade, is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD. Taq DNA Polymerase also accepts modified deoxyribonucleoside triphosphates as substrates for highly efficient DNA labeling with radionucleotides, digoxigenin, fluorescein, or biotin.
Application
• Taq DNA Polymerase, GMP Grade, can be applied for:
• PCR
• RT- PCR
• Other primer-extension reactions, such as sequencing and labeling
包装
1 kit containing 2 components
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文献和实验for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended
High Specificity PCR Amplification Using AccuPrime Taq DNA Polymerase
Taq DNA Polymerase 0.25 µl 0.5 µl 1 µl Autoclaved distilled water To 10 µl To 25 µl
Dideoxy Sequencing Reactions Using Taq Polymerase
Tuq DNA polymerase is a highly stable polymerase isolated from the thermophilic organism Thermus aquaticus . Because of its unique properties this enzyme has been extensively used for amplification of DNA fragments by the polymerase chain
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