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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见产品外包装
- 英文名:
Poly(dA)
- 库存:
该产品已停产
- 供应商:
嵘崴达
- CAS号:
见产品外包装
- 保存条件:
−20°C
- 规格:
5 UNITS
Poly(dA)
Poly-deoxy-adenylic acid
General description
Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units. Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.
Application
Poly(dA) is suitable for use in:
• DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies
• as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis
• in Pyrococcus abyssi DNA polymerase assay
Sequence
Chain Length Mean chain length lot 20-17: 250-500 bases.
Unit Definition
1 A260 unit corresponds to 40 μg ssDNA.
| 10223581001 | Poly (dA) | 5 A260 units |
| 10736988001 | Creatine Kinase (CK), 500mg | 500 mg |
| 10737275001 | HK/G6P-DH, 30 mg | 30 mg (10 ml) |
| 4673484001 | FastStart SYBR Green Master 5 荧光定量pcr酶 | 5 ml (4 x 1.25 ml) (for 200 reactions of 50 µl final reaction volume) |
| 11583786001 | DTT, 25g | 25 g |
| 10127825001 | HK/G6P-DH, 15 mg | 15 mg (5 ml) |
| 10223565001 | Histone 组蛋白 | 10 mg |
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文献和实验Screening Poly [dA/dT()] cDNA for Gene Identification
The goal for developing the SPGI (screening poly [da/dT(−)] cDNA for gene identification technique was to generate an efficient tool for maximal identification of the expressed genes from eukaryotic genomes. The impetus for developing
多聚-β-羟丁酸poly-β-hydroxybutyricacid
几种芽孢杆菌属(Bacillus),假单胞菌属(Pseudomonas)、螺菌属(Spirillum)、固氮菌属(Azotobacter)以及其他许多种细菌在菌体内制造的贮藏物质。 在光学显微镜下可以因折光或呈颗粒状而被观察到,并可用苏丹黑染色。在细菌中作为代替油脂贮藏物,而合成此化合物。一般在培养基中氮源缺乏而碳源多的条件下合成,如果供给氮化合物,水解成单体的β-羟丁酸后,经过乙酰乙酸、乙酰CoA,被利用来合成生物体内各种成分
we synthesized three primers which contain (dT)17 and either (dA) or (dC) or (dG) at the 3' end. We reasoned that the presence of these three bases at the 3' end would 'anchor' the primers at the upstream end of the poly(A) tail and allow sequencing
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