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文献和实验Synthesis and Probing of Membrane-bound Peptide Arrays
Synthesis and Probing of Membrane-bound Peptide Arrays Ronald Frank Department of Chemical Biology, GBF (German Research Center for Biotechnology), 38124 Braunschweig, Germany
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
buffers: NaCl stepwise elution buffers are generatedby combining Solutions A and B in various ratios (for instance,5% Solution B for 0.1 M NaCl; 15% Solution B for 0.3 MNaCl; 25% Solution B for 0.5 M NaCl; 35% Solution B for 0.7M NaCl; 50% solution B
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
kinaseassay using pGS2 peptide. GSK3b activity is presented in a graph as a percentage ofuntreated cells (normalised; 100% activity from control cells equates to 52 pmol/min/mg). n.s. , non-significant; *, p 2. Comparative analysis of phosphorylation
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