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Neobioscience
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文献和实验Terminal Deoxynucleotidyl Transferase
-stranded DNA fragments. In addition, incorporation can be limited to a single nucleotide by using [alpha-32 P]cordycepin-5´- triphosphate. This analog lacks a free 3´ hydroxyl group, preventing incorporation of additional nucleotides. 点击浏览该文件
Bst-catalyzed radiolabeled DNA sequencing
analog, 7-deaza-dGTP, but differed in their deoxy/dideoxynucleotide ratios to yield fragments ranging in size from the beginning of the polylinker to greater than 300 bases from the primer, or fragments from about 150 to greater than 600 bases
Appendix H: Radioactive tracers
as a calibrated output, either through the use of a digital or analog meter, or as a series of clicks , by conversion of the current through a speaker. The sensitivity of the system depends on the voltage applied between the electric plates. Since alpha
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