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- invitrogen
- I1203
- 2025年07月13日
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文献和实验Measurement of [Ca2+]i in Cell Suspensions Using Indo-1
Intracellular calcium, in particular the cytosolic free ionized calcium concentration [Ca2+ ]i , is tightly regulated under physiological conditions. Stimulation of receptors, belonging to almost all the classes so far described, will result
Apoptosis detection methodology is an ever evolving science. The caspase family of cysteine proteases plays a central role in this environmentally conserved mechanism of regulated cell death. New methods that allow for the improved detection
Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
, which cleaved the ester bond and left the calcium-sensitive free acid trapped within the cell (5 ). A number of improved calcium indicators have since been developed (e.g., fura-2, indo-1, and fluo-3), but the basic principles of dye loading and continuous
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