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货号:A1048002
品牌:invitrogen
规格:5 ML
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文献和实验Growing Cells in Geltrex Reduced Growth Factor Basement Membrane Matrix
objects are ready for use. 4. Thin Gel Method (non-gelling) for hESC Applications: 1) Thaw Geltrex. See Precautions. 2) Mix Geltrex by slowly pipetting solution up and down; be careful not to introduce air
Chondrogenic Differentiation of hESC in Micromass Culture
Human adult cartilage has limited capacity for self-renewal. Accordingly, repair of cartilage tissue damaged as a result of acute traumatic injury or via chronic wear or degenerative disease, such as arthritis, is a major clinical problem
Culture of Human Embryonic Stem Cells (hESC)
at the same density. Immediately before plating hESC, MEFs are rinsed once or twice with PBS. hESC are plated onto MEFs as small clumps in 2.5ml per well of hESC medium containing 4ng/ml bFGF (R&D Systems Cat #233-FB). Cells are fed every day until ready
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