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- 2025年07月14日
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文献和实验Cryopreservation and Recovery of Mature Differentiated Neural Cells
实验试剂 Neurobasal® Medium B-27® Serum-Free Supplement GlutaMAX™-I Trypan Blue Synth-a-Freeze® Cryopreservation Medium 实验设备 Cryogenic Vials Isopropanol Chamber
General Protocol to Prepare Neuronal Cultures
pellet. Resuspend pellet in 1 ml of the provided B27/Neurobasal/0.5 mM glutamine medium. Resuspend cells by gently pipetting up and down. For E18 Hippocampus, media includes 25uM glutamate. 11. Aliquot 20 μl and mix with 20 μl of 0.4% trypan blue
General Protocol for Astrocytes
. After 4-6 days astrocytes will be nearly confluent and ready to harvest or pass. If desired, you can change 50% of the medium to Neurobasal / 2% B27/0.5mM glutamine in preparation to harvest the next day for transfer to neuron cultures. If expansion
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