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货号:4392938
产品名称:FG,KIT RNA-TO-CT 1-STEP TAQMAN,200 RXN
品牌:abi
关键字:abi,Applied Biosystems公司,4392938,FG,KIT RNA-TO-CT 1-STEP TAQMAN,200 RXN
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文献和实验SINGLE STEP PROKARYOTIC RNA ISOLATION
In the past, poly(A)+ RNA has not been detected in prokaryotes. In the early 80s, a new method of isolating RNA from bacteria was developed, involving lysis by protease K in the presence of SDS and potent RNase inhibitors (heparin & 1,10
BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
was frozen overnight. 9、The following ribonuclease treatment step reduces the amount of RNA present in the final BAC preparation. After thawing, centrifuge at 10K for 10 minutes in the SS34rotor. Then, the supernatant of each tube is transfered
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
. 注意事项 1. All steps performed at room temperature. 2. RNA can be stored at –80°C for short time. 3. At step 8, it is needed to prolong the treated time for cell >5 x 106.
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