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货号:4392653
产品名称:FG,KIT RNA-TO-CT 1-STEP TAQMAN,40 RXN
品牌:abi
关键字:abi,Applied Biosystems公司,4392653,FG,KIT RNA-TO-CT 1-STEP TAQMAN,40 RXN
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文献和实验SINGLE STEP PROKARYOTIC RNA ISOLATION
In the past, poly(A)+ RNA has not been detected in prokaryotes. In the early 80s, a new method of isolating RNA from bacteria was developed, involving lysis by protease K in the presence of SDS and potent RNase inhibitors (heparin & 1,10
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
. 注意事项 1. All steps performed at room temperature. 2. RNA can be stored at –80°C for short time. 3. At step 8, it is needed to prolong the treated time for cell >5 x 106.
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
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