OBS,FG, Human GRP78 (HSPA5)

Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures

with chaperones in these cells is of interest. Furthermore, Pb and Cu bind strongly to the molecular chaperone heat shock 70 kDa protein Hspa5, also known as glucose-regulated protein 78 (Grp78) or immunoglobulin-binding protein (BiP). This chapter describes

【求助】关于BAPTA的图片

化。BAPTA的pKa3为5.47，pKa4为6.36。这个特点显示了去质子化的步骤并不包含在Ca的螯合步骤中。由于不受质子的干扰，它的螯合率比EGTA要高很多。BAPTA-AM是BAPTA的乙酰甲酯衍生物，通过AM法，能被轻易负载到细胞中。BAPTA-AM对于细胞内钙离子的监控是非常有用的。 kaige88 葡萄糖调节蛋白（glucose regulated proteins, GRPs）是内质网中一类重要的驻留蛋白，包括GRP78和GRP94。 GRP

【求助】有关shRNA抑制NF2基因的问题

expression in targeting cells. To construct the hairpin siRNA expression cassette, complementary DNA oligonucleotides for siRNA of human NF-2 (NF-2 sh1 and NF2 sh2) were synthesized, annealed, and inserted into FG12. 请问下为什么要制作2条 NF-2 shRNA(NF