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- 4379876
- 2025年07月03日
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货号：4379876
产品名称：FG,10X PCR Buffer I, 2 x 75ML
品牌：abi
关键字：abi，Applied Biosystems公司，4379876，FG,10X PCR Buffer I, 2 x 75ML
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文献和实验Clonality - X Chromosome Inactivation Assay
(Life Technologies) 10X PCR buffer (100 mM Tris-HCL pH 8.3, 500 mM KCL, 15 mM MgCl2, 0,01 % w/v gelatin, autoclaved) (Perkin Elmer) dNTP, each 10 mM (Perkin Elmer) 7-deaza dGTP, 10 mM (Boehringer Mannheim) DMSO, minimum 99.5%, GC (Sigma
Mouse p27 PCR Using Gitschier Buffer
with H2O and aliquot into 1.5 mL eppendorf tubes PCR Reaction Mix (To make a fresh master mix, multiply # PCR reactions x volumes, below) 2 µL KG-1 (10x) 1x 2 µL KG-2 (10x) 1x 2 µL Primer 1 (1uM) 0.1 mM 2 µL Primer 2 (1uM) 0.1 mM
of H2O:54 g of Tris base27.5 g of boric acid20 ml of 0.5 M EDTA (pH 8.0)The 0.5x working solution is 45 mM Tris-borate/1 mM EDTA.TBE is usually made and stored as a 5x or 10x stock solution. The pH of the concentrated stock buffer should be approx
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