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- abi
- 4379878
- 2025年07月11日
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货号：4379878
产品名称：FG,10X PCR Buffer II & MgCl2, 2x75ML
品牌：abi
关键字：abi，Applied Biosystems公司，4379878，FG,10X PCR Buffer II & MgCl2, 2x75ML
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文献和实验Clonality - X Chromosome Inactivation Assay
(Life Technologies) 10X PCR buffer (100 mM Tris-HCL pH 8.3, 500 mM KCL, 15 mM MgCl2, 0,01 % w/v gelatin, autoclaved) (Perkin Elmer) dNTP, each 10 mM (Perkin Elmer) 7-deaza dGTP, 10 mM (Boehringer Mannheim) DMSO, minimum 99.5%, GC (Sigma
Mouse p27 PCR Using Gitschier Buffer
with H2O and aliquot into 1.5 mL eppendorf tubes PCR Reaction Mix (To make a fresh master mix, multiply # PCR reactions x volumes, below) 2 µL KG-1 (10x) 1x 2 µL KG-2 (10x) 1x 2 µL Primer 1 (1uM) 0.1 mM 2 µL Primer 2 (1uM) 0.1 mM
Troubleshooting for PCR and multiplex PCR
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.* The 10x PCR buffer contains: 500 mM KCl; 100 mM Tris-HCl (pH 8.3); 15 mM MgCl2 (the final concentrations
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