C CPG 100 UMOL/G 1G

Methylation Analysis of CpG Islands

and have distinct properties when compared to the rest of the genome. CpG islands are often located in the promoter region or the first exon of expressed genes and show a high G + C content (60/270%), the remainder of the genomic DNA has a G + C content of 40

Methylated CpG Island Amplification

and 2 volumes 100% ETOH. Store at �70°  C for 1 hour and centrifuge 30 min. at >10,000 g. Pour the ETOH out, air dry the pellets. Resuspend in 10-20 m l TE and determine DNA concentration using a spectro-photometer. 2.1.2 Ligation of Adapter

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

to 37°C and 65°C Sonicator Spectrophotometer Thermal cycler, automated Timer Tubes, microcentrifuge, 1.5-mL Tubes, polypropylene, 14-mL (e.g., 17- x 100-mm) for use in high-speed centrifuge Tubes, polypropylene, 50-mL