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- abi
- GEN901055
- 2025年07月13日
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货号：GEN901055
产品名称：A CPG 100 UMOL/G 1G
品牌：abi
关键字：abi，Applied Biosystems公司，GEN901055，A CPG 100 UMOL/G 1G
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文献和实验Methylated CpG Island Amplification
hybridization solution (e.g. BLOTTO) Random-primed DNA labeling kit Wash solutions (Wash1 2xSSC, 0.1%SDS; Wash2 0.1XSSC, 0.1%SDS) 1.2. RDA and Cloning PCR Products 3 X EE buffer : 30 mM EPPS (SIGMA) pH 8.0, 3 mM
Methylated CpG Island Amplification (MCA)
(for precipitation and quantitation when dNTPs are present) 100% ETOH Agarose gel electrophoresis reagents Filter hybridization reagents: 96 pin replicator system (Nunc) Nylon membranes DNA hybridization solution (e.g
of BSA of 1, 5, 7.5 and 10 µ g/ml. Prepare a blank of NaCl only. Prepare a series of sample dilutions. Add 100 µ l of each of the above to separate tubes (use microcentrifuge tubes) and add 1.0 ml of Coomasie Blue to each tube.
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