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货号:403078
产品名称:AFLP I PRESELECT PRIMER MIX
品牌:abi
关键字:abi,Applied Biosystems公司,403078,AFLP I PRESELECT PRIMER MIX
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文献和实验(Mse1/EcoR1) 1ul AFLP Core Mix 15ul * AFLP Core Mix 配方: ddH 2 O 13.8ul MgCl 2 1.6ul dNTPs(2.5mM) 1.6ul Taq酶(1U/ul) 1ul 10×Buffer 2ul
, but are of defined length for a given primer. The sequence of the extension can also vary from one primer to another but is of a single, defined sequence within a given primer. The selective nature of AFLP-PCR is based on the 3' extensions on the oligonucleotide
P in this reaction and still get useful primer b) incubate at 37C for 30 mins To Label AFLP ladder (from Gibco/BRL) a) mix: 2 ul H2O 1 ul 10X rxn buffer (NEB) 4 ul AFLP ladder 2 ul 33P-ATP 1 ul T
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