Figure 1: Mouse liver extract was transferred onto PVDF membrane and first probed for actin, then stripped with Western ReProbe™ and subsequently screened for tubulin antigens. Tubulin band was developed without loss of signal or background problems.
应用: l 对照蛋白的抗体剥离再检测
l 在同一个免疫印迹中比较磷酸化和总蛋白
l 对于不满意的免疫印迹进行再分析和校准
l 节省珍贵的样本和试剂
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