检测不同的一抗 无需浪费珍贵的样品重新制胶电泳来检测不同的一抗。只需使用ReProbe抗体剥离缓冲液简单地处理膜,即可除去第一种一抗。通常此步骤只需30分钟,耗时依赖于一抗的亲和性大小。在抗体剥离之后,可以使用一种新的一抗进行重新检测(见图)。请注意ReProbe不推荐用于使用TMB,氯萘酚和DAB等底物显色的免疫印迹。产品提供5X的溶液,使用15-20ml对于一个标准的免疫印迹(7.5 x 8.5cm)
Figure 1: Mouse liver extract was transferred onto PVDF membrane and first probed for actin, then stripped with Western ReProbe™ and subsequently screened for tubulin antigens. Tubulin band was developed without loss of signal or background problems.
应用:
对照蛋白的抗体剥离再检测
在同一个免疫印迹中比较磷酸化和总蛋白
对于不满意的免疫印迹进行再分析和校准
节省珍贵的样本和试剂
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