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hellma
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101-10-40/Z600172/111-10-40/Z600717
| 规格: | 101-10-40/Z600172 | 产品价格: | ¥1700.0 |
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| 规格: | 111-10-40/Z600717 | 产品价格: | ¥2300.0 |









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文献和实验5.5 Direct labeling of PCR products and homogenous assays 101 5.6 In vitro expression of PCR product 103 Protocol 5.1: Cycle sequencing – Applied Biosystems Big Dye terminators 108 Chapter 6 Purification and Cloning of PCR Products 111 6.1 Introduction
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
). The recipient strain (R1236/B211) can be denser than the donor (#111/B428). Mix 200 ul of each strain. Incubate at 37o C without agitation for 20 min to 1 hr. Plate as 100 ul aliquots onto LB Tet3 Kan40 Cm34. Do the Control: Spot











