FLUO-3,AM ESTER,100UG 50013

膜透性形式，因此可以通过孵化进入细胞。由于AM eater相对低的水溶性，温和的洗涤剂Pluronic F-127常被用作分散剂以促进载入细胞。Fluo-3/AM本身不结合Ca2+，一旦染料进入细胞立即被内源性酯酶水解成fluo-3。Fluo-3/AM的一个重要新兴应用是它在高通量药物筛选方面的使用。

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca²⁺, but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening. For information on Fluo-3, see catalog number 50010.

Because of the relatively low water solubility of the AM ester, Pluronic® F-127 (cat# 59004), a mild detergent, is often used as a dispersing agent to facilitate dye loading. See the product information sheet for more information.

• Orange red solid soluble in DMSO
• Store -20°C and protect from light, especially when in solution
• C₅₁H₅₀Cl₂N₂O₂₃
• MW: 1129.9
• Abs/Em (after hydrolysis): 506/526 nm (low or high [Ca²⁺] )
• ε (after hydrolysis): 86,000 M^-1 cm^-1 (506 nm)
• K_d (Ca²⁺) (after hydrolysis): 450 nM
• [121714-22-5]

BAPTA-based ion indicators like Fluo-3 have been shown to be fixable in situ by EDC/EDAC (cat# 59002). The fixation of indicator dyes is useful for downstream immunofluorescence and IHC studies (Cell Calcium 1997, 21(3), 175).

As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed. The organic anion transporter inhibitor Probenecid (#50027) can be used to slow the rate of indicator efflux from cells.

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