AM4-65 70039

AM4-65是可固定的红色荧光神经末端探针。染料光谱和SynaptoRed C2 (也叫 FM4-64)相同，但是前者在分子的亲水末端具有额外的胺基，使得染料可被甲醛等固定。

AM4-64 is a fixable, activity-dependent fluorescent nerve terminal probe. AM4-64 functions similarly as SynaptoRed™ C2 (70021) (equivalent to FM®4-64), but with the additional property of being fixable. Thus, AM4-64 is a useful tool for synapse studies where subsequent fluorescent immunochemistry is desired. AM4-64 and FM®4-64 have identical excitation and emission wavelengths.

• λ_Ex/λ_Em (membranes) = ~510/750 nm*
• Dark purple solid soluble in water
• MW: 555.5

* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed™ C2 (FM®4-64), and should be applicable to AM4-64.

Nerve Terminal Dyes and Kits

Product	Catalog No.	Features
SynaptGreen™ Dyes (Ex/Em ~480/660 nm in membranes)	70020... 70053	View full list of SynaptoGreen™ Dyes
SynaptoRed Dyes™ (Ex/Em ~510/750 nm in membranes)	70021... 70050	View full list of SynaptoRed™ Dyes
Background Reducers
ADVASEP-7	70029	Sulfonated cyclodextrin that aids in removal of free dye during washes
SCAS	70037	Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7
Sulforhodamine 101	80101	Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes
Nerve Terminal Staining Kits
Nerve Terminal Staining Kit I	70030	Includes SynaptoGreen™ C4 and ADVASEP-7
Nerve Terminal Staining Kit II (A)	70031	Includes AM1-43 and ADVASEP-7
Nerve Terminal Staining Kit II (B)	70031-1	Includes AM1-43 and SCAS
Nerve Terminal Staining Kit III	70032	Includes SynaptoGreen™ C4 and Sulforhodamine 101
Nerve Terminal Staining Kit V	70034	Includes SynaptoRed™ C2 and ADVASEP-7

m is the number of carbons in the lipophilic tail and n is the number of double bonds linking the two aromatic rings in the dye.
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.