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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20 °C
- 保质期:
1年
- 英文名:
CF790 succinimidyl ester 92155
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
CF® Dye SE/TFP Esters are amine-reactive forms of Biotium’s bright and photostable CF® Dyes. The succinimidyl ester (SE) group, also known as N-hydroxysuccinimide (NHS) esters, on the dye molecule reacts with primary amines such as lysine residues of antibodies or proteins, amine-modified oligonucleotides and other amine-containing molecules to form a stable amide linkage. CF® Dye TFP (tetrafluorophenyl) esters are more stable alternatives to succinimidyl ester. CF® Dye TFP esters are available for CF®850 and CF®870, Biotium’s industry-leading near-infrared dyes with emission above 850 nm.
- Amine-reactive fluorescent dyes with high labeling efficiency
- Label antibodies, proteins, amine-modified oligonucleotides and other amine-containing molecules.
- Offered with Biotium’s bright and photostable CF® Dyes which provide superior signal-to-noise
- Available in 36 CF® Dye colors from blue to near-IR wavelengths
- Near-IR CF®850 and CF®870 TFP esters offer emission above 850 nm
Due to Biotium’s unique manufacturing and packaging process, our amine-reactive CF® Dyes are provided with the highest purity and more stable ester dye forms than other vendors. As a result, our CF® Dye SE/TFP products retain more reactivity and provide a higher labeling efficiency for users.
Superior CF® Dyes
CF® Dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability, and water solubility compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.
Also see our CF® Dye SE Protein Labeling Kits and CF® Dye Mix-n-Stain™Antibody Labeling Kits.
CF® Dye SE/TFP Ester
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
Materials 1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃ 2.GTP,100 mM stock,titrate to pH ~7.Need 2 ml. 3.Waterbath,set at 37℃ 4.SS34 rotor,5 ml and 15 ml tubes and adaptors.Bring the rotor,adaptors,and tubes
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for day 0. 2.0.5 mM ATP,0.2 mM CaCl2,70 mM KCl,150 mM PIPES,pH 7.0,1 ml. 3.16 mM ATP,0.2 mM CaCl2,5 mM DTT,5 mM lysine,10 mM glutamic acid,20 mM Tris-HCl,pH 8.0,10 ml
cross-linker, 1-pyrenebutanoic acid, succinimidyl ester. The technique effectively tethers the enzyme to the carbon nanotube which enhances bioelectrocatalysis, preserves the conductive nature of the carbon surface, and facilities direct electron











