TPEN (FULL NAME: TETRAKIS-(2-P

TPEN是重金属螯合剂，不会影响 Ca++, Mg++ , Na+ 或K+的浓度。

TPEN (Tetrakis-(2-pyridylmethyl)ethylenediamine) is an excellent heavy metal chelator while it does not affect Ca² , Mg² , Na , or K concentrations. Thus, TPEN is a useful tool to identify effects of heavy metals (Zn² , Fe² /Fe³ , Cu² , and Mn² , etc.) on fluorescent indicators of Ca² , Mg² , Na , or K .

• Off-white solid soluble in ethanol, DMSO or DMF
• Store at 4°C
• C₂₆H₂₈N₆
• MW: 425
• [16858-02-9]

As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed. The organic anion transporter inhibitor Probenecid (#50027) can be used to slow the rate of indicator efflux from cells.