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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
EDC (EDAC, 1-(3-DIMETHYLAMINOPROPYL-3 59002
- 库存:
99
- 供应商:
Biotium
- CAS号:
25952-53-8
EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.
- White solid soluble in water
- Store desiccated at -20°C
- C8H18ClN3
- MW: 191.7
- [25952-53-8]
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文献和实验Determination of Protein Contacts by Chemical Cross‐Linking With EDC and Mass Spectrometry
) 0.2 M [1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride] (EDC; Pierce) in 50 mM MES; prepare immediately before use 0.02 M sulfo‐N‐hydroxysuccinimide (Sulfo
. Functional shells for subsequent coupling reactions are provided by generating organic shells providing amine and carboxyl groups at the nanoparticles’ shell surface. Conjugation of proteins to the nanoparticles is achieved using N -(3-dimethylaminopropyl)-N
IASYS-binding cuvette and getting KD
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)in 15 ml of deionized H2O to get 0.077 g/ml (0.400 mol/L)concentration and aliquot into 250 µL volume and store in a -20 ℃ freezer. (3) Thaw one vial of each NHS and EDC. (4) Incubate the cuvette












