- 询价
- Biotium
- 美国
- 30028-2
- 2025年07月15日
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- 详细信息
- 技术资料
- 保存条件：
-70°C
- 保质期：
6个月
- 英文名：
Steady-Luc Firefly HTS Assay Kit (100 ml) 30028-2
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
The Steady-Luc™ HTS assay system is a homogeneous, high-sensitivity firefly luciferase reporter gene assay kit. It is a glow-type assay with signal half-life of about 3 hours, allowing batch processing in microplates.
Features
- Simple, single step homogeneous assay
- Luminescence half life of about 3 hours
- Great value: save up to 45% compared to other brands
Kit Components
- D-Luciferin
- Steady-Luc™ Firefly Assay Buffer
Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.
Biotium’s Steady-Luc™ Firefly Assay Buffer contains a proprietary mixture of substances that prevent the adenyl-oxyluciferin reaction, producing a long lasting, glow-type signal. It produces luminescence signal with a half-life of about 3 hours, for a high-throughput microplate assay.
Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.
Biotium also offers Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) (catalog no. 30028L), a new packaging format that includes lyophilized assay buffer for convenient room temperature shipping and storage at -20°C.
More Luciferase Kits & Related Products
| Catalog number | Product | Description |
|---|---|---|
| 30085 | Firefly Luciferase Assay Kit 2.0 | Flash-type firefly luciferase assay kit. |
| 30075 | Firefly Luciferase Assay Kit (Lyophilized) | Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. |
| 99923 | 5X Firefly Lysis Buffer | Extra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well. |
| 30028 | Steady-Luc™ Firefly HTS Assay Kit | Glow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates. |
| 30028L | Steady-Luc Firefly HTS Assay Kit (Lyophilized) | Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. |
| 30082 | Renilla Luciferase Assay Kit 2.0 | Flash-type Renilla luciferase assay kit. |
| 99912 | 5X Passive Lysis Buffer | Extra lysis buffer for use with Renilla Luciferase Assay Kits, only required if using well sizes larger than 24-well. |
| 30081 | Firefly and Renilla Luciferase Single Tube Assay Kit | Assay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube. |
| 99821-100mL | 1X Passive Lysis Buffer 2.0 | Extra lysis buffer for use with Firefly & Renilla Single Tube Assay Kits, only required if using well sizes larger than 24-well. |
| 30020 | ATP-Glo™ Bioluminometric Cell Viability Assay Kit | Flash-type luminescence-based assay for measuring cell viability or ATP concentration. |
| 22003 | Mini Cell Scrapers | Mini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates. |
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