- 询价
- Biotium
- 美国
- 30028-L1
- 2025年07月16日
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- 详细信息
- 技术资料
- 保存条件：
-70°C
- 保质期：
6个月
- 英文名：
Steady-Luc Firefly HTS Assay Kit (Lyophilized) 30028-L1
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
The Steady-Luc™ HTS assay system is a homogeneous, high-sensitivity firefly luciferase reporter gene assay kit. It is a glow-type assay with signal half-life of about 3 hours, allowing batch processing in microplates.
Features
- Simple, single step homogeneous assay
- Luminescence half life of about 3 hours
- Lyophilized assay buffer for convenient shipping & storage
- Great value: save up to 45% compared to other brands
Kit Components
- D-Luciferin
- Steady-Luc™ Firefly Assay Buffer (Lyophilized)
- Reconstitution Buffer
Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.
Biotium’s Steady-Luc™ Firefly Assay Buffer contains a proprietary mixture of substances that prevent the adenyl-oxyluciferin reaction, producing a long lasting, glow-type signal. It produces luminescence signal with a half-life of about 3 hours, for a high-throughput microplate assay.
Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.
This product is comparable to our original Steady-Luc™ HTS Assay (catalog no. 30028), but with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. Simply dissolve the lyophilized assay buffer in the supplied reconstitution buffer before use.
More Luciferase Kits & Related Products
| Catalog number | Product | Description |
|---|---|---|
| 30085 | Firefly Luciferase Assay Kit 2.0 | Flash-type firefly luciferase assay kit. |
| 30075 | Firefly Luciferase Assay Kit (Lyophilized) | Firefly luciferase assay kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. |
| 99923 | 5X Firefly Lysis Buffer | Extra lysis buffer for use with Firefly Luciferase Assay Kits, only required if using well sizes larger than 24-well. |
| 30028 | Steady-Luc™ Firefly HTS Assay Kit | Glow-type firefly luciferase assay with signal half-life of about 3 hours for high-throughput screening in multi-well plates. |
| 30028L | Steady-Luc Firefly HTS Assay Kit (Lyophilized) | Steady-Luc kit with lyophilized assay buffer for economical room temperature shipping and convenient storage at -20°C. |
| 30082 | Renilla Luciferase Assay Kit 2.0 | Flash-type Renilla luciferase assay kit. |
| 99912 | 5X Passive Lysis Buffer | Extra lysis buffer for use with Renilla Luciferase Assay Kits, only required if using well sizes larger than 24-well. |
| 30081 | Firefly and Renilla Luciferase Single Tube Assay Kit | Assay kit for measuring firefly and Renilla activities sequentially in the same sample in a single tube. |
| 99821-100mL | 1X Passive Lysis Buffer 2.0 | Extra lysis buffer for use with Firefly & Renilla Single Tube Assay Kits, only required if using well sizes larger than 24-well. |
| 30020 | ATP-Glo™ Bioluminometric Cell Viability Assay Kit | Flash-type luminescence-based assay for measuring cell viability or ATP concentration. |
| 22003 | Mini Cell Scrapers | Mini-cell scrapers, useful for lysing cells in 96-well, 48-well, or 24-well plates. |
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