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- Biotium
- 美国
- 10011
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
X-Gal (5-bromo-4-chloro-3-indolyl-b-galactopyranoside)
- 库存：
99
- 供应商：
Biotium
- 保存条件：
-20°C
X-gal (5-Bromo-4-chloro-3-indolyl-β-galactopyranoside) is the most widely used chromogenic substrate for β-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (maximum absorbance 615 nm).
- White solid soluble in DMSO
- Store desiccated at -20°C and protect from light
- C14H15BrClNO6
- MW: 408.6
- [7240-90-6]
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文献和实验, transfect the DNA into mammalian cells, and then fix and stain a tissue with X-gal. The cells that turn blue must have the beta galactosidase enzyme, the product of the lac Z gene, which could only have been expressed from the donated promoter. Ergo
Materials Fixative 2% Paraformaldehyde (in PBS) Staining solution 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, 2 mM MgCI, 0.01% sodium deoxycholate 0.02% Nonidet P-40 (NP-40) in PBS. X-gal Stock 40 mg
Detection of -Galactosidase Activity: X-gal Staining
X-gal staining is a rapid and convenient histochemical technique used to detect reporter gene expression. A prerequisite is the creation or acquisition of transgenic reporter mouse lines, in which the bacterial LacZ gene has been knocked
