- 询价
- Biotium
- 美国
- 10012
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 英文名：
RED-BETA-D-GAL (5-BROMO-6-CHLORO-3-INDOL 10012
- 库存：
99
- 供应商：
Biotium
- CAS号：
93863-88-8
Red-β-D-Gal (5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-gal, but generates a red or magenta (λmax ~ 565 nm) colored precipitate on enzyme hydrolysis.
- White solid soluble in DMSO
- Store at 4°C and protect from light
- C14H15BrClNO6
- MW: 408.6
- [93863-88-8]
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文献和实验Beta-Gal staining in primary neurons
2 Part b. X-gal (5-bromo-4-chloro-3-indolyl-beta-gal) 40 mg/ml in DMSO Heat part a & b to 37 C Add part b to part a to final 1 mg/ml X-gal. Add part b slowly while mixing, or crystals will form. Filter to remove crystals.
Cell Staining for Senescence-Associated beta-Galactosidase (SA-beta-Gal) Activity
in DMF (CAUTION! see Hint #1) 20 mg/ml X-Gal (5-bromo-4-chloro-3-indoly Â-D-galactopyranoside) Store -20°C X-Gal is not stable in water and should be added to the Staining Solution just before use 100 mM Potassium Ferricyanide 100 mM Potassium
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
