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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4°C
- 英文名:
RHODAMINE 110, BIS-(L-ASPARTIC ACID AMID 10210
- 库存:
99
- 供应商:
Biotium
D2R (Rhodamine 110, bis-(L-aspartic acid amide), ditrifluoroacetic acid salt) is a general substrate for caspases including caspases 3 and 7. Unlike other fluorogenic substrates for caspases, D2R appears to be membrane-permeant, and thus can be used to measure caspase activity in live cells without osmotic shock for flow cytometry studies, as well as for cell extracts. For real-time detection of caspase-3 activity in intact cells, see our novel NucView®488 Caspase-3 Substrate.
- Ex/Em of end product (R110) = 496/520 nm
- Off-white to pink solid soluble in DMSO
- Store at 4°C and protect from light
- C32H26F6N4O13
- MW: 788.57
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文献和实验were labeled with Hoechst 33342 (H1399, H3570, H21492), tetramethylrhodamine ethyl ester (TMRE, T669) and rhodamine 110, bis-L-aspartic acid amide (R22122) for 15 minutes. Apoptotic cells show green cytosolic fluorescence resulting from cleavage
to hydrogen peroxide, cells were labeled with Hoechst 33342 (H1399, H3570, H21492), tetramethylrhodamine ethyl ester (TMRE, T669) and rhodamine 110, bis-L-aspartic acid amide (R22122) for 15 minutes. Apoptotic cells show green cytosolic fluorescence resulting
Acids Glycine 75 50 0.667 L-Alanine 89 25 0.281 L-Arginine 211 105 0.498 L-Asparagine-H2O 150 50 0.333 L-Aspartic acid 133 30 0.226 L-Cysteine hydrochloride-H2O 176 100 0.568 L-Cystine 2HCl 313 31 0.0990 L-












