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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
(1:3 dilution of stock) 0.375 M Tris-HCl (pH 8.8) 0.1 % SDS Stacking Gel (Final Concentrations) 3 % acrylamide (1:10 dilution of stock) 0.125 M Tris-HCl (pH 6.8) 0.1% SDS Electrode
Protein Gel Preparation and Staining
Tris Base pH to 8.8 with HCl 0.4% SDS 0.4 g SDS up to 100 ml with Q 4X Stacking Gel Buffer 0.5 M Tris pH 6.8 6.06 g Tris Base pH to 6.8 with HCl 0.4% SDS 0.4 g SDS up to 100 ml with Q 1X Glycine Running Buffer
SDS-polyacrylamide gel electrophoresis
Glycice: 94 g SDS: 5 g H2 O upto1 liter 4x Resolving-gel buffer 1.5M Tris-Cl (pH 8.8) 8x Stacking-gel buffer 1M Tris-Cl (pH
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