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文献和实验Replication timing by density transfer
volume of a 2x enzyme mix that contains the appropriate restriction enzyme buffer, restriction enzyme (usually 1 microliter per sample) and 0.25 microgram/ml RNase A. If the DNA prep is clean, the digest can go overnight at 37 degrees C.] Choosing
buffer 2.5% Triton X-100 Protease analysis; renatures enzymes after electrophoresis 10x zymogram development buffer 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5 mM CaCl2, 0.02% Brij-35 Protease analysis; activates enzymes after electrophoresis Premixed
Mouse p27 PCR Using Gitschier Buffer
with H2O and aliquot into 1.5 mL eppendorf tubes PCR Reaction Mix (To make a fresh master mix, multiply # PCR reactions x volumes, below) 2 µL KG-1 (10x) 1x 2 µL KG-2 (10x) 1x 2 µL Primer 1 (1uM) 0.1 mM 2 µL Primer 2 (1uM) 0.1 mM
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