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文献和实验10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
of H2O:54 g of Tris base27.5 g of boric acid20 ml of 0.5 M EDTA (pH 8.0)The 0.5x working solution is 45 mM Tris-borate/1 mM EDTA.TBE is usually made and stored as a 5x or 10x stock solution. The pH of the concentrated stock buffer should be approx
). 8. Freeze-thaw 3x (10'freeze-10'thaw). 9. Spin down debris @ 14K RPM for 5'. 10. Transfer 100 µl supernatant to a new set of tubes. 11. Set new set of tubes and add 10 µl of supernatant and 50 µl buffer I in each.
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