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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
TS Biotin ethylenediamine (biotin ethylenediamine thiosulfate,sodium salt)
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
- 保存条件：
-20°C
TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH. However, unlike the MTS group , which is neutral, TS is negatively charged and thus probes bearing a TS group are ideal for specifically labeling thiols exposed at the exterior cell surface because charged probes are less likely to enter cells.
It’s available in 3 variations: Biotin, Biotin-X, and Biotin-XX. The X and XX spacer should facilitate the interaction between biotin and avidin or streptavidin.
TS Biotin ethylenediamine
- Biotin ethylenediamine thiosulfate, sodium salt
- C14H23N4NaO6S3
- MW: 462.54
- Off-white solid soluble in DMSO or water
- Store desiccated at -20°C
TS Biotin-X ethylenediamine
- Biotin-X ethylenediamine thiosulfate, sodium salt
- C20H34N5NaO7S3
- MW: 575.7
- Off-white solid soluble in DMSO or water
- Store desiccated at -20°C
TS Biotin-XX ethylenediamine
- Biotin-XX ethylenediamine thiosulfate, sodium salt
- C26H45N6NaO8S3
- MW: 688.86
- Off-white solid soluble in DMSO or water
- Store desiccated at -20°C
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文献和实验). 2. Stacking buffer (4×): 0.5 M Tris-HCl, pH 6.8, and 0.4%(w/v) SDS. Store at RT. 3. 30% acrylamide/bis solution (37.5:1 with 2.6% C) andN , N , N , N ￠-tetramethyl-ethylenediamine (TEMED; Bio-Rad,Hercules, CA). 4. Ammonium persulfate: prepare 10% (w/v
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
carcinoma cells are culturedin Dulbecco’s Modified Eagle’s Medium (DMEM)supplemented with 10% fetal bovine serum under standardtissue culture conditions. 2. A solution of trypsin (0.25%) and ethylenediamine tetra aceticacid (EDTA) (1 mM) is prepared
Site-directed hydroxy radical mapping of nucleosome positions in vitro
. However, an equally effective identical EDTA reagent activated instead with 2-pyridine is available commercially from Toronto Research Chemicals (TRC Inc, Toronto, Canada) as N-[S-(2-Pyridylthio)cysteaminyl]ethylenediamine-N,N,N',N'-tetraacetic acid (prduct P996250
