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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer (5-CR110-PEO4, SE)
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
- 保存条件:
-20°C避光
5-CR110-PEO4 SE (full name: 5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled. Carboxyrhodamine 110 and its derivatives are, in general, significantly more photostable than any other known green fluorescent dyes including Alexa Fluor® 488. In addition, their spectra are unaffected by pH change from 4 to 9. Unlike Alexa Fluor® 488, which is readily degraded under alkaline conditions, CR110 and its derivatives are highly stable under both acidic and basic conditions. As a result, CR110 and its derivatives are superior alternatives to other green fluorescent dyes for applications where only a single dye is required for the labeling.
- λEx/λEm (MeOH) = 502/524 nm
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C36H38N4O12
- MW: 718.71
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文献和实验SE 活性部分远低于50%(AlexaFluor488微量标记试剂盒产品信息,Invitrogen)。相反,所有Biotium 的CF染料具有相对稳定的胺活性的SE基团型,这比大部分AlexaFluor 染料的SE更耐水解。总体来说,CF染料SE产品一贯的给出更高的标记效率,提供给用户更好的使用价值。选择方案:蓝色(350-450nm处激发) CF 350、Alexa Fluor 350、AMCA等----亮蓝和紫外光激发CF350是类似于Alexa Fluor 350和传统荧光染料AMCA的蓝色
and IRDyes®等严重的磺化作用有较强的吸湿性,恶化水解作用等问题。所有Biotium的CF染料具有相对稳定的胺活性的SE基团型,这比大部分AlexaFluor 染料的SE更耐水解。总体来说,CF染料SE产品一贯的给出更高的标记效率,提供给用户更好的使用价值。 二、选择方案: 1、蓝色(350-450nm处激发) CF 350、Alexa Fluor 350、AMCA等----亮蓝和紫外光激发。 CF350是类似于Alexa Fluor 350和传统荧光染料AMCA的蓝色荧光染料,CF350
-strand breaks with TAL effector nucleases. Genetics 186(2):757–761. doi: 10.1534/genetics.110. 120717 , genetics.110.120717 [pii] 11. Reyon D, Tsai SQ, Khayter C, Foden JA, SanderJD, Joung JK (2012) FLASH assembly ofTALENs for high-throughput genome













