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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
6-TAMRA, SE in DMSO at 90mg/mL (0.17M) 90097
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
6-TAMRA SE (full name: 6-Carboxytetramethylrhodamine succinimidyl ester) is the amine-reactive form of 6-TAMRA single isomer (90035). Supplied at 90 mg/mL (0.17 M) in anhydrous DMSO. The solution is suitable for labeling oligonucleotides.
- λEx/λEm (MeOH) = 540/565 nm
- Dark red solution
- Store desiccated at -20°C and protect from light
- C29H25N3O7
- MW: 527.5
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文献和实验很大部分紫外线光,导致细胞凋亡不理想。 3. 用含40mL PBS(RT)的50 mL锥形管中洗NPC,室温离心(1200 转/min,7min),缓慢减速,弃掉上清液。 4. 室温条件下在5 mL 0 .1 M PBS溶液中重悬NPC。 5. 向NPC中加入5mL sta 5(6)-TAMRA,SE(Invitrogen公司),立即涡旋, 37ºC避光孵育15min。 6. 用40 mL预冷的0.1M PBS洗3次(如步骤3)。
, although some hypotheseshave been suggested. The first hypothesis proposes lithium as amagnesium (Mg 2+ )-competitive inhibition of GSK-3 (16) , whereasthe second suggests lithium inhibitor by potassium deprivation (17) . Lithium is a nonspecific GSK-3 inhibitor
200 μL with a 0.45 μm filter and inject 20 μL. 4) MALDI: Mix 1 μL of ˜ 20 mg/mL peptoid with 1 μL matrix. Spot 1 μL on MALDI plate and allow to air dry. Matrix and acquisition mode is dependent on sample (Fig. 5).











