- 询价
- Biotium
- 美国
- 29088-100ug
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
6个月
- 英文名：
Annexin V (His Tag) 29088-100ug 29088-100ug
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Recombinant Human Annexin V (His Tag) expressed from E. coli as a single, non-glycosylated polypeptide chain containing 320 amino acids with a molecular weight of 36.8 kDa.
Annexin V is a member of the annexin family of calcium-dependent phospholipid binding proteins with high affinity for phosphatidylserine (PS). In viable cells, PS is predominantly located on the inner leaflet of the plasma membrane. During apoptosis, PS translocates from the inner leaflet to the outer leaflet. Fluorescently labeled Annexin V can bind to PS located on the outer leaflet and therefore is commonly used as a marker of apoptosis. Annexin V has also been identified as an anticoagulant protein that is involved in the blood coagulation cascade through indirect inhibition of the thromboplastin-specific complex.
We also offer Annexin V Conjugates and Annexin V Conjugates, Azide-Free and Lyophilized in colors ranging from UV to far-red for live cell staining. Also view our Annexin V Near-IR CF® Dye Conjugates (also lyophilized and preservative-free), compatible with small animal NIR in vivo imaging. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.
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文献和实验染料，它不能透过完整的细胞膜，但在凋亡中晚期的细胞和死细胞，PI能够透过细胞膜而使细胞核红染。因此将Annexin-V与PI匹配使用，就可以将凋亡早晚期的细胞以及死细胞区分开来。方法1、悬浮细胞的染色：将正常培养和诱导凋亡的悬浮细胞（0.5~1×106）用PBS洗2次，加入100ul Binding Buffer和FITC标记的Annexin-V（20ug/ml）10ul,室温避光30min,再加入PI（50ug/ml）5ul,避光反应5min后，加入400ul Binding Buffer，立即
, PI)是一种核酸染料，它不能透过完整的细胞膜，但在凋亡中晚期的细胞和死细胞，PI能够透过细胞膜而使细胞核红染。因此将Annexin-V与PI匹配使用，就可以将凋亡早晚期的细胞以及死细胞区分开来。 方法 1 悬浮细胞的染色 将正常培养和诱导凋亡的悬浮细胞（0.5~1×106）用PBS洗2次，加入100ul Binding Buffer和FITC标记的Annexin-V（20ug/ml）10ul，室温避光30min，再加入PI（50ug/ml）5ul，避光反应5min后，加入400ul
Purification of Annexin V and Its Use in the Detection of Apoptotic Cells
, and the second using a single step purification protocol via a glutathione S-transferase (GST) tag. Labeling of the resulting annexin V with a fluorescent dye to allow visualization of the protein is also explained. Finally, two methods are described
