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- Biotium
- 美国
- 29047
- 2026年01月06日
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- 保存条件：
4°C 避光
- 保质期：
6个月
- 英文名：
Annexin V, CF790 29047
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
CF® Dye Annexin V Conjugates can be used to stain the surface of apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.
- Azide-free for no-wash, real-time live cell imaging in culture medium
- Fast & simple detection of phosphatidylserine on apoptotic cells
- Choice of 11 CF® dye colors with superior brightness & photostability
- For real-time live cell imaging, fluorescence microscopy, or flow cytometry
Preservative Free for Real-Time, Live Cell Imaging
Annexin V conjugates typically are supplied as stock solutions with azide as a preservative for end-point staining assays in Annexin V binding buffer. Our azide-free CF® Dye Annexin V Conjugates are supplied as lyophilized solids with no azide or other preservatives that might be incompatible with live cell imaging. After reconstitution in buffer, the conjugates can be added to cell culture medium for no-wash, real-time live cell imaging. Our Mini Syringe Filters are convenient for small volume sterile filtration of azide-free Annexin V stock solutions or other aqueous solutions for use in cell culture.
See Annexin V staining in real time:
Imaging of apoptosis in staurosporine-treated HeLa cells stained with CF®594 Annexin V (red, apoptotic cell surface probe) and Oxazole Yellow (green, dead cell nuclear stain). All nuclei are stained blue with Hoechst.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
More Apoptosis Assays
We also offer Near-IR CF® Dye Annexin V Conjugates, preservative-free and lyophilized, compatible with small animal in vivo imaging. Annexin V Conjugate solutions (with azide) also are available with a large selection of CF® Dyes, biotin, R-PE, APC, and other labels. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.
CF® Dye Annexin V Conjugates, Azide-Free, Lyophilized
| Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®350 | 347/448 nm | 5 ug | 29012-5ug | CF®350 Features |
| CF®405M | 408/452 nm | 5 ug | 29009-5ug | CF®405M Features |
| CF®450 | 450/538 nm | 5 ug | 29083R-5ug | CF®450 Features |
| CF®488A | 490/515 nm | 5 ug | 29005R-5ug | CF®488A Features |
| CF®555 | 555/565 nm | 5 ug | 29004R-5ug | CF®555 Features |
| CF®568 | 562/583 nm | 5 ug | 29010R-5ug | CF®568 Features |
| CF®583R | 586/609 nm | 5 ug | 29085R-5ug | CF®583R Features |
| CF®594 | 593/614 nm | 5 ug | 29011R-5ug | CF®594 Features |
| CF®633 | 630/650 nm | 5 ug | 29008R-5ug | CF®633 Features |
| CF®640R | 642/662 nm | 5 ug | 29014R-5ug | CF®640R Features |
| CF®647 | 650/665 nm | 5 ug | 29003R-5ug | CF®647 Features |
| CF®660R | 663/682 nm | 5 ug | 29069R-5ug | CF®660R Features |
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文献和实验Q：Annexin V 凋亡检测试剂盒的原理是什么？ A：Annexin V 是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 PS 有高度亲和力，可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合，将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，核染料 PI 或 7-AAD
示例： Jurkat细胞用1μM喜树碱(Camptothecin) (左)或未加药 (右)处理4h，FITC-Annexin V/PI荧光双染细胞凋亡检测试剂盒染色后，流式细胞仪荧光检测。 Annexin V-FITC单阳性细胞为早期凋亡细胞，Annexin V-FITC和PI染色双阳性的细胞为坏死细胞或者晚期凋亡。PI单染色阳性位裸核细胞。 实测数据可能会因细胞类型、细胞凋亡情况，检测仪器等的不同而存在差异，图中数据仅供参考。 流式检测细胞凋亡的数据分析方法： 1） 选中所有颗粒，将FSC
磷脂酰丝氨酸（Phosphatidylserine, PS）正常位于细胞膜的内侧，但在细胞凋亡的早期，PS可从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中（图3）。Annexin-V是一种分子量为35~36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力特异性结合。将Annexin-V进行荧光素（FITC、PE）或biotin标记，以标记了的Annexin-V作为荧光探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生。碘化丙啶（propidine iodide, PI）是一种核酸
