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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL 20210
- 靶点:
FITC
- 浓度:
2 mg/mL
- 应用范围:
Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- 宿主:
Mouse
- 保质期:
6个月
- 目录编号:
20210
- 库存:
99
- 供应商:
Biotium
- 标记物:
CF488A
- 克隆性:
单克隆
- 保存条件:
-20°C
- 形态:
Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide
This is a monoclonal mouse anti-fluorescein (FITC) antibody labeled with our superior CF® dyes.
- Available in 6 bright and photostable CF® dyes
- Suitable for western, immunofluorescence, and immunohistology in FFPE tissues
See our full selection of anti-tag and anti-hapten antibody conjugates.
Note: Conjugates of blue fluorescent dyes like CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Monoclonal Mouse Anti-Fluorescein (FITC)
| Conjugation | Ex/Em | Size | Catalog No. |
|---|---|---|---|
| CF®405M | 408/452 nm | 50 uL (100 ug) | 20214-1 |
| 0.25 mL (500 ug) | 20214 | ||
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20210-1 |
| 0.25 mL (500 ug) | 20210 | ||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20211-1 |
| 0.25 mL (500 ug) | 20211 | ||
| CF®633 | 630/650 nm | 50 uL (100 ug) | 20212-1 |
| 0.25 mL (500 ug) | 20212 | ||
| CF®640R | 642/662 nm | 50 uL (100 ug) | 20213-1 |
| 0.25 mL (500 ug) | 20213 | ||
| CF®660R | 663/682 nm | 50 uL (100 ug) | 20399-50uL |
| 0.25 mL (500 ug) | 20399-0.25mL |
View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
CF® dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
CF is a registered trademark of Biotium, Inc.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验min。②荧光素的准备 根据欲标记的蛋白质总量,按每毫克免疫球蛋白加0.01mg荧光色素,用分析天平准确称取所需的异硫氰酸荧光素粉末。也可用下述公式计算出免疫球蛋白、荧光素的量,还可以算出需加缓冲液的量。a.蛋白溶液:含量Amg/m1;容积Bml。b.总蛋白量(AXB)=Crag。c.C/20~C/10=Dmg(如蛋白含量低于20mg/ml,用C/10;如高于20mg/ml,用C/20)。d.荧光素FITC的量:(1/50~2/100)XC=Emg。e.巳0.5mol/L(pH9.5)碳酸盐缓冲
的检测。 (二) 操作步骤 将纯化的IgG抗体对PH9~9.5碳酸盐缓冲液透析过夜, 透析后抗体液移入小烧杯中 ↓ 称取适量IFTC,加入二甲亚砜(DMSO)(FITC~1mg/1ml DMSO) 使终浓度为1mgFITC/1mlDMSO FITC/IgG比例:如IgG浓度为1mg/ml,FITC/IgG比例约为50μgFITC/mgIgG; 如IgG为5~10mg/ml,则比例为25
荧光素FITC标记抗体的方法(Marsshall法、Chadwick法、改良法、透析标记法)
总蛋白量(AXB)=Crag。 c.C/20~C/10=Dmg(如蛋白含量低于20mg/mL,用C/10;如高于20mg/mL,用C/20)。 d.荧光素FITC的量:(1/50~2/100)×C=Emg。 e.巳0.5mol/L(pH9.5)碳酸盐缓冲液D/10=FmL。 f. PBS量D-(B+F)=GmL。 注:A为蛋白含量,mg/mL;B为蛋白质溶液的容积;C为蛋白总量,mg;D为常数,mg;正为荧光素的量,mg;F为碳酸












