Concanavalin A, CF633 conjugat

CoA是最广泛使用的外源凝集素，能够选择性地结合α-吡喃甘露糖或α-吡喃葡萄糖残基。

Concanavalin A (Con A) is a widely used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are commonly found in the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.

• Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
• Detect glycoconjugates in microscopy and flow cytometry
• Stain glycoproteins in gels
• Withstands fixation and permeabilization
• Choice of 10 CF® dyes from UV to near-infrared
• Superior CF® dyes are bright, photostable, and water-soluble

Lectins are also versatile probes for detecting glycoconjugates in microscopy and flow cytometric applications and for gel staining of glycoproteins. In neutral and alkaline solutions, Con A exists as a tetramer with a molecular weight of approximately 104 kDa. In acidic solutions (pH below 5.0), Con A exists as a dimer. Con A can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway.

Find the Right Stain for Your Application

Con A and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Concanavalin A Dye Conjugates

Product	Conjugation	Ex/Em	Size	Catalog No.	Purchase
CF®350 Concanavalin A (Con A)	CF®350	347/448 nm	5 mg	29015	Purchase 29015
CF®405S Concanavalin A (Con A)	CF®405S	404/431 nm	5 mg	29075	Purchase 29075
CF®405M Concanavalin A (Con A)	CF®405M	408/452 nm	5 mg	29074	Purchase 29074
CF®488A Concanavalin A (Con A)	CF®488A	490/515 nm	5 mg	29016	Purchase 29016
CF®594 Concanavalin A (Con A)	CF®594	593/614 nm	5 mg	29017	Purchase 29017
CF®633 Concanavalin A (Con A)	CF®633	630/650 nm	5 mg	29018	Purchase 29018
CF®640R Concanavalin A (Con A)	CF®640R	642/662 nm	5 mg	29019	Purchase 29019
CF®680 Concanavalin A (Con A)	CF®680	681/698 nm	5 mg	29020	Purchase 29020
CF®750 Concanavalin A (Con A)	CF®750	755/777 nm	5 mg	29080	Purchase 29080
CF®770 Concanavalin A (Con A)	CF®770	770/797 nm	5 mg	29058	Purchase 29058

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.