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文献和实验Labeling DNA Breaks In Situ by Klenow Enzyme
by primer extension. Of these applications, end-labeling DNA with a 5′-overhang by Klenow has been developed into an in situ technique for the detection of DNA breaks using incorporation of modified nucleotides that may then be detected and visualized
Food-Grade Corynebacteria for Enzyme Production
microorganisms. To solve this problem, here we describe a general method for the construction of engineered corynebacteria bearing a single copy of a gene coding for a hydrolytic enzyme or a desired protein in its chromosome where it is stably maintained
Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
is incubated in the presence of one or more deoxyribonucleotide triphosphates (one of which is labeled with 32 P in the a-phosphate group) and the Klenow fragment of DNA polymerase. As in the case with the kinase labeling of DNA (Chapter 39 ), the DNA fragment
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