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文献和实验Primary Amplification of Genomic DNA using DOP - PCR
Boehringer Mannheim, 1051482 Primer “UN1” Midland Certified Reagent Co. Telenius 6MW [5’-CCGACTCGAGNNNNNNATGTGG-3’] Taq DNA polymerase, 5 U/μl Fermentas Life Sciences, Cat. Epo282 500U TAE buffer, 10X Advanced Biotechnologies, Cat. no.08-514-001
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
to the concentration of 100 mM. Store at -20°C. 6. T4 DNA ligase and ligation reaction buffer (Promega). Storeat ?20°C. 7. PCR grade water or Millipore purified water. 8. Biolase Taq DNA polymerase (Bioline USA Inc.). Store at?20°C. 9. 10× PCR
Detection of Viruses in Infected Plant Extracts using Immunocapture-PCR
, 2.5 U Taq DNA Polymerase and 50 pmoles of each primer (degenerate). Overlay reaction mix with PCR-grade, sterile mineral oil and subject to amplification using a programme of: 5 min at 94o C, followed by 40 cycles of 1 min at 940 C, 1 min at 550 C
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