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- 2026年01月02日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Methylated-DNA IP Kit
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
-20
HIGHLIGHTS
- Robust enrichment & immunoprecipitation of DNA containing 5mC.
- Includes a highly specific anti-5-methylcytosine monoclonal antibody for defined, reproducible results.
- Eluted, ultra-pure DNA is ideal for use in subsequent molecular-based analyses (e.g., assembling genomic libraries and determining genome-wide methylation status).
DESCRIPTION
The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
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文献和实验• 以96孔板方式，从同一个细胞或组织样本中同时纯化基因组 DNA和总RNA • 处理 96 个样本，只需不到60 分钟 • 从同一样本中纯化高品质的基因组DNA和总RNA • 96孔板方式，高效的操作流程 • 高度标准化的操作流程 • 高重复性的DNA和RNA产量 Product description The AllPrep DNA/RNA 96 Kit is well suited for high
Methylated DNA Immunoprecipitation (MeDIP)
Introduction Methylation of cytosines can mediate epigenetic gene silencing and is the most prominent DNA modification in eukaryotes. MeDIP is an immunocapturing approach to enrich DNA that is methylated. The principle
PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction: 1 x 95 °C, 2 min 21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min 1 x 72
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