- 询价
- Biotium
- 20076
- 美国
- 2025年11月15日
- Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- Donkey
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
驴抗人
- 抗体英文名：
CF633 Donkey anti-human IgG(H+L), highly cross-adsorbed, 2 mg/mL
- 靶点：
Human IgG
- 浓度：
2 mg/mL
- 应用范围：
Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- 宿主：
Donkey
- 保质期：
6个月
- 目录编号：
20076
- 库存：
99
- 供应商：
Biotium
- 标记物：
CF633
- 克隆性：
多克隆
- 保存条件：
-20°C
- 形态：
Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution
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文献和实验Analysis of Oligosaccharide Ligands by High Perfor
protein Protocol: Coating IgG or IgG-chimera to HPLAC-A column 1. Dialyze IgG or IgG-chimera with PBS buffer. The protein concentration is adjusted to 0.5-1 mg/ml PBS. One ml of packed column binds about 10 mg of IgG or IgG
Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography
chimeric protein Protocol: Coating IgG or IgG-chimera to HPLAC-A column 1. Dialyze IgG or IgG-chimera with PBS buffer. The protein concentration is adjusted to 0.5-1 mg/ml PBS. One ml of packed column binds about 10 mg
), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG
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