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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
驴抗人
- 抗体英文名:
CF633 Donkey anti-human IgG(H+L), highly cross-adsorbed
- 靶点:
Human IgG
- 浓度:
2 mg/mL
- 应用范围:
Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- 宿主:
Donkey
- 保质期:
6个月
- 目录编号:
20076-1mg
- 库存:
99
- 供应商:
Biotium
- 标记物:
CF633
- 克隆性:
多克隆
- 保存条件:
-20°C
- 形态:
Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution
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文献和实验Analysis of Oligosaccharide Ligands by High Perfor
from the total IgG passed through the column. Chemical Cross-linking IgG to the HPLAC A column (optional) 1. Keep HPLAC-A column at 10�, and wash it with 10 column volumes of borate buffer at a flow rate of 0.5 ml/min. 2. Pass 10 column volumes of TEA
Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography
of IgG or IgG chimera. 2. Condition the HPLAC-A column on a conventional HPLC system with the same PBS buffer at a flow rate of approximately 0.5 ml/min for 30 min at l0�. 3. Pass the solution through the column at a flow rate
µl PlateBlock™ for 2 h 4. Aspirate or tap the plate (do not wash) 5. Stabilization with Liquid Plate Sealer® animal-free for 2 - 15 min 6. Aspirate or tap the plate (do not wash), dry and store 7. Dilute the patient sample (1:100) in LowCross
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