AccuClear超高灵敏度双链DNA定量溶液 (1000

AccuClear超高灵敏度dsDNA定量试剂利用新型的下一代DNA结合染料，高灵敏准确定量纯化后不同浓度范围的dsDNA样品。微孔板上每次的分析线性从0.03ng-250ng。与吸光度为基础的方法不同，AccuClear染料高选择性结合双链DNA优于单链DNA或RNA。AccuCleardsDNA定量分析适用于配制激发和吸收滤光片检测绿色荧光的96孔板荧光仪。AccuClear染料独特的光谱特性使其非常适用于带有蓝色LED激发光源的设备。Biotium的470 Mini荧光计也可用于AccuClear分析。AccuClear也兼容手持式荧光计如Invitrogen的Qubit® a和 Promega的QuantiFluor®-P，但是这些仪器的标准曲线可能不能全部涵盖AccuClear试剂盒标准曲线的全部动态范围。试剂盒包含AccuClear染料，1X缓冲液，不含标准品，用户可自己准备DNA标准品。

The AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified DNA samples, designed for use with fluorescence microplate readers. AccuClear® is our most popular DNA quantitation kit, with a very wide linear detection range from 0.03 – 250 ng. This solution does not come with a DNA standard; see the AccuClear® Ultra High Sensitivity dsDNA Quantitation Kits (31028&31029) which contains calf thymus DNA standards.

Features

• Biotium’s most popular & versatile DNA quantitation assay
• Extremely wide linear range of 0.03-250 ng DNA
• Highly specific for dsDNA
• Designed for fluorescence microplate readers
• Green fluorescence emission

Kit Components

• AccuClear® Dye, 100X
• DNA Quantitation Buffer, 20X Concentrate*

*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit Name	DNA or RNA	Dye Ex/Em (nm)	Suggested Instrument	Features
AccuGreen™ High Sensitivity DNA	DNA	502/523	Qubit® fluorometer	Compare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNA	DNA	500/530	Qubit® fluorometer	Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNA	DNA	468/507	Fluorescent Plate Reader	Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR * Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity DNA	DNA	468/507	Fluorescent Plate Reader	Versatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNA	DNA	485/530	Fluorescent Plate Reader	Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNA	DNA	350/460	Fluorescent Plate Reader	Broad linear range with blue fluorescence
AccuBlue® Broad Range RNA	RNA	650/670	Fluorescent Plate Reader or Qubit® fluorometer	The widest linear range of available RNA quantitation kits, with exceptional accuracy, sensitivity, and high RNA selectivity