AccuBlue高灵敏度双链DNA定量溶液 31008

AccuBlue高灵敏度dsDNA定量试剂盒极其灵敏并选择性检测dsDNA，将其他污染物的影响降到最低。试剂盒比Quant-iT宽检测限dsDNA和其他传统的检测试剂盒更优越，操作方便，更安全。高灵敏度试剂是细胞膜不透性的，而PicoGreen或高灵敏度Quant-iTDNA结合染料能很容易透过细胞膜。另外，试剂非常稳定。试剂盒试剂盒包含1X定量缓冲液，100X增强液。AccuBlue可以4℃保存，而Quant-iT试剂必须分装保存避免冻融。试剂盒兼容微型光谱仪，分光光度计和手持式迷你荧光计。试剂盒提供线性检测范围0.2-100ng足够在96孔板上1000次分析的试剂。

The AccuBlue® High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0.2 – 100 ng. This solution does not come with a DNA standard; see the AccuBlue® High Sensitivity Kit (31006), which contains a set of calf thymus DNA standards.

Features

• Highly specific for dsDNA
• Uses the non-toxic, non-mutagenic EvaGreen® dye for safer handling and easy disposal
• Linear detection range from 0.2-100 ng
• Designed for fluorescence microplate readers
• Green fluorescence emission

Kit Components

• AccuBlue® High Sensitivity dsDNA Quantitation Solution, 1X
• AccuBlue® High Sensitivity Enhancer, 100X

The AccuBlue® High Sensitivity dsDNA Quantitation Assay uses non-toxic, non-mutagenic EvaGreen® dye (download the EvaGreen Safety Report for more information), making it safer than PicoGreen® or Quant-iT® kits.

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit	DNA or RNA	Detection range (in assay)*	Dye Ex/Em (nm)	Suggested instrument	Features
AccuGreen™ High Sensitivity DNA	DNA	0.1-100 ng	502/523	Qubit® fluorometer	Compare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNA	DNA	2-1000 ng	500/530	Qubit® fluorometer	Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNA	DNA	1-3000 pg**	468/507	Fluorescence microplate reader	Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR
AccuClear® Ultra High Sensitivity DNA	DNA	0.03-250 ng	468/507	Fluorescence microplate reader	Versatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNA	DNA	0.2-100 ng	485/530	Fluorescence microplate reader	Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNA	DNA	2-2000 ng	350/460	Fluorescence microplate reader	Broad linear range with blue fluorescence
AccuBlue® Broad Range RNA	RNA	5-1000 ng	650/670	Fluorescence microplate reader or Qubit® fluorometer	The widest linear range of available RNA quantitation kits Exceptional accuracy, sensitivity, and high RNA selectivity

* Standard assay volume is 200 uL
** AccuBlue® NextGen limit of detection is in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and PicoGreen are registered trademarks of Thermo Fisher Scientific.