AccuBlue宽量程双链DNA定量溶液 31009

AccuBlue宽检测限dsDNA定量试剂盒极其灵敏并选择性检测dsDNA，将其他污染物的影响降到最低。试剂盒比Quant-iT宽检测限dsDNA和其他传统的检测试剂盒更优越，具有相似的检测限，但是更方便，并且提高了动态检测范围。试剂盒包含1X定量缓冲液，100X染色液，100X增强液。AccuBlue可以4℃保存，而Quant-iT试剂必须分装保存避免冻融。试剂盒兼容微型光谱仪，分光光度计和手持式迷你荧光计。试剂盒提供线性检测范围2-2000ng足够在96孔板上1000次分析的试剂。

The AccuBlue® Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a linear detection range from 2 – 2000 ng and is designed for use with fluorescence microplate readers. This solution does not come with a DNA standard; see the AccuBlue® Broad Range Kit (31007) which contains a set of calf thymus DNA standards.

Features

• Highly specific for dsDNA
• Broad linear detection range, from 2-2000 ng
• Designed for fluorescence microplate readers
• Blue fluorescence emission

Kit Components

• AccuBlue® Broad Range Dye, 100X
• AccuBlue® Broad Range Buffer, 1X
• AccuBlue® Broad Range Enhancer, 100X

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit	DNA or RNA	Detection range (in assay)*	Dye Ex/Em (nm)	Suggested instrument	Features
AccuGreen™ High Sensitivity DNA	DNA	0.1-100 ng	502/523	Qubit® fluorometer	Compare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNA	DNA	2-1000 ng	500/530	Qubit® fluorometer	Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNA	DNA	1-3000 pg**	468/507	Fluorescence microplate reader	Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR
AccuClear® Ultra High Sensitivity DNA	DNA	0.03-250 ng	468/507	Fluorescence microplate reader	Versatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNA	DNA	0.2-100 ng	485/530	Fluorescence microplate reader	Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNA	DNA	2-2000 ng	350/460	Fluorescence microplate reader	Broad linear range with blue fluorescence
AccuBlue® Broad Range RNA	RNA	5-1000 ng	650/670	Fluorescence microplate reader or Qubit® fluorometer	The widest linear range of available RNA quantitation kits Exceptional accuracy, sensitivity, and high RNA selectivity

* Standard assay volume is 200 uL
** AccuBlue® NextGen limit of detection is in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.