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- 保存条件:
-20°C
- 英文名:
rhodamine-DHPE 60026
- 库存:
99
- 供应商:
Biotium
- CAS号:
126111-99-7]
Rhodamine-DHPE (N-(Lissamine rhodamine B sulfonyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) is a rhodamine-labeled glycerophosphoethanolamine lipid. DHPE conjugates of rhodamine dyes have been used as fluorescence energy acceptors in combination with NBDPE as the donor in membrane fusion assays using the principle of fluorescence energy transfer (FRET) (1-3). These probes have also been used for following membrane trafficking during endocytosis (4,5).
- λEx/λEm (MeOH) = 560/581 nm
- Dark red solid soluble in chloroform
- Store at -20°C and protect from light
- C70H117N4O14PS2
- MW: 1333.80
- [126111-99-7]
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文献和实验Conjugation of Antibodies to Fluorescein or Rhodamine
The isothiocyanate derivative of the fluorophore, fluorescein or rhodamine, is coupled to the amino groups of IgG antibody in a one-step procedure and excess label is removed by gel filtration.
Rhodamine-Phalloidin/Calcofluor Staining
Rhodamine-Phalloidin/Calcofluor Staining David Amberg Grow 50 mls of yeast to 5x10E6. Add formadehyde to the media to 4% (33 mls. of 10%). Fix in media at temperature for 10 min. Spin down cells 2-3 K for 5 min
Nystatin-Rhodamine B Assay for Estimating Activity of Killer Toxin from Kluyveromyces lactis
Killer toxin activity is commonly determined by the well test (1 ), which ensures the inhibition zone on a substratum of sensitive strain cells. The Rhodamine B assay described in Chapter 31 is based on estimation of the fraction of killed
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