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Recombinant Human Transforming

Growth Factor-Beta 1
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  • ¥1080 - 21735
  • PROSPEC
  • 以色列
  • cyt-716
  • 2025年12月28日
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      TGFB1

    • 库存

      部分小规格有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      2ug/10ug/100ug

    规格:2ug产品价格:¥1080.0
    规格:10ug产品价格:¥2415.0
    规格:100ug产品价格:¥21735.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-716

    SYNONYMS

    Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

    INTRODUCTION

    Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.

    DESCRIPTION

    TGFB1 Human Recombinant produced in CHO cells is a glycosylated homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25.6kDa. The TGFB1 is purified by proprietary chromatographic techniques.

    SOURCE

    CHO cells.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid (TFA) And trehalose (1:20 protein to Trehalose ratio).

    SOLUBILITY

    It is recommended to reconstitute the lyophilized TGFB1 in sterile 10mM HCl at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions. 

    STABILITY

    Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    PURITY

    Greater than 95.0% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.

    BIOLOGICAL ACTIVITY

    The ED50 as determined by the dose-dependent inhibition of IL-4-induced proliferation of HT-2 cells is 0.0149ng/ml, corresponding to a specific activity of 6.7x107units/mg.

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    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    该产品被引用文献

    de Caestecker, M., Piek, E., & Roberts, A. B. (2021). Role of transforming growth factor-betas in human disease. Handbook of Experimental Pharmacology, 267, 1-51.

    Akhurst, R. J., & Hata, A. (2012). Targeting the TGFβ signalling pathway in disease. Nature Reviews Drug Discovery, 11(10), 790-811.

    Chen, G., Deng, C., & Li, Y. P. (2019). TGF-β and BMP signaling in osteoblast differentiation and bone formation. International Journal of Biological Sciences, 15(11), 2132-2142.

    Varelas, X. (2014). The Hippo pathway effectors TAZ and YAP in development, homeostasis and disease. Development, 141(8), 1614-1626.

    Chen, C. C., & Lau, L. F. (2009). Functions and mechanisms of action of CCN matricellular proteins. International Journal of Biochemistry and Cell Biology, 41(4), 771-783.

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    文献支持
    Recombinant Human Transforming Growth Factor-Beta 1
    ¥1080 - 21735