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- 2025年07月09日
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文献和实验Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
for each well. View Image Figure 13.6.2 Schematic representation of the multiplexed peptide–nuclear receptor interaction assay at a fixed
both equilibration steps; however, it is not necessary. 4. Treat cells with agonist. (typically 5-25'). 5. Quickly aspirate media and add fixative (~ 2 ml/well). Fix for 15-20'. 6. Wash 3x. 7. Aspirate the final wash, taking care to dry the edges
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
Table 4.4.1 Materials Interaction Trap Plasmids a a , b b Interaction Trap Plasmids Interaction Trap Yeast Selection Strains f Interaction Trap Yeast Selection Strains
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